![]() This implies that the full number of HA-33 ![]() The hemagglutination activity of the 650-kDa L-TC was determined to be titer of 2 7, while no hemagglutination titer could be detected for the other TC species. HA-17 Complexes in the L-TC-We performed in vitro reconstitution of the mature L-TC by adding increasing amounts of HA-33.In an earlier study, based on negative stain electron microscopy of the serotype A BoNT and analysis of a separated non-toxic complex, Boroff et al. This is therefore a novel observation regarding the structure of the botulinum TC. No TC image with four or more arms was observed in the field of view. The number of “arm” attachments with ∼10 nm length increased with the size of the L-TC species the average number of arms was: one (84.4%, n = 209), two (73.3%, n = 172), and three (55.6%, n = 135) for 540-kDa L-TC, 610-kDa L-TC, and 650-kDa L-TC, respectively. HA-70 complex via the HA-70 molecule, as shown in Fig.HA-17 complexes that adhered to the M-TC.Interestingly, subsequent L-TC species revealed unique “arm” attachments that appeared to be rod-like particle. ![]() This may be supported by the observation during differential scanning calorimetry, that the isolated HA-70 molecules exhibited no distinct melting transition through the range 10 to 100 ☌ (data not shown). It is noteworthy that the HA-70 molecule appears to have no rigid configuration. 2, A-2), suggesting blocks building up sequentially to form a complex.
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